WNT proteins are extracellular signaling molecules involved in the control of embryonic development.
This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities.
This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains.
It may be involved in mesoderm segmentation.
This protein is found to be present in fish, amphibia and mammals.
The deduced 379aa WIF1 secreted protein contains an N-terminal signal sequence, a 150aa WIF domain, 5 epidermal growth factor (EGF)-like repeats that are similar to those of tenascin, and a C-terminal hydrophilic domain of approximately 45aa.
Overexpression of human WIF1 in frog embryos affected somitogenesis depending on the site and timing of WIF1 injection.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
