The TNFAIP2 gene was induced in HUVECs in response to the proinflammatory molecules TNF, IL1B , and lipopolysaccharide.
TNFAIP2 demonstrated the characteristics of a primary response gene in its rapid and substantial induction in the absence of intermediary protein synthesis.
The authors found that TNFAIP2 transcription was induced during capillary tube formation in vitro.
Northern blot analysis detected a 4.2kb human TNFAIP2 transcript.
During mouse development, expression of the Tnfaip2 transcript varied in a temporal fashion that was organ-specific.
The TNFAIP2 cDNA encodes a predicted 654aa protein with a single N-linked glycosylation site; stretches of glutamates, lysines, and alanines near the N-terminal; and 4 glutamines near the C-terminal.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
