TRPM4 has 1040aa and contains 6 putative transmembrane domains.
Northern blot analysis detected TRPM4 expression in most adult tissues tested, with highest levels in heart, prostate, and colon.
In fetus, TRPM4 expression was most abundant in kidney.
There were at least 2 distinct bands detected (6.2 and 4.2kb), indicating alternative splicing of TRPM4.
In addition, a smaller but relatively abundant 2.4kb transcript was detected in testis.
The shorter protein results from alternative splicing, with the third and fourth exons being spliced out and the beginning of the first exon being truncated.
As a consequence, the first in-frame methionine lies in the fifth exon, resulting in the deletion of the first 174aa.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
