TOPBP1 is a binding protein which interacts with the C-terminal region of topoisomerase II beta.
This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.The deduced 1522aa protein has a calculated molecular mass of about 171kD.
TOPBP1 contains 8 repeating regions throughout its sequence that share similarity with repeat regions 1 and 2 of Rad4.
It also has an auto-ADP-ribosylation site and 2 C-terminal nuclear localization signals.
The 3-prime untranslated region contains 12 AUUUA mRNA-instability motifs.
Northern blot analysis detected ubiquitous expression, with smearing characteristic of unstable mRNA.
Transcripts above 10kb were detected only in heart.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
