NR2C2, act as ligand-activated transcription factors.
The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C-terminal.
The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes.
The cDNAs encode a predicted 615aa human protein and a 596aa rat protein that are 98% identical.
The TR4 sequence is similar to that of the TR2 orphan.
Together they appear to form a distinct subfamily.
The 2 transcripts appeared to differ in the length of the 3-prime untranslated region.
In mouse and rat testis, TR4 was expressed most abundantly in spermatocytes.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
