NR2C1 is a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a C-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily.
This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes.
Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.Dot blot hybridization indicated that TR2-5 mRNA levels increased after castration of rats and that this increase was reversed by 5-alpha-dihydrotestosterone injection.
Expression of TR2-5 resulted in a 52kD protein.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
