While investigating radiation-induced gene expression in mouse hepatocellular carcinoma cells, Fukuda et al.
isolated a clone showing similarity to Sdf2, and they obtained the full-length mouse cDNA.
The deduced human and mouse proteins contain 221aa and share 88% sequence identity.
SDF2L1 has an N-terminal hydrophobic signal sequence and an endoplasmic reticulum retention-like motif at the C terminus.
SDF2 and SDF2L1 show significant similarity to the central hydrophilic regions of POMT1, POMT2 , the O-mannosyltransferase family of S.
cerevisiae and C.
albicans, and the rt protein of Drosophila.
Northern blot analysis detected ubiquitous expression of Sdf2l1 in mouse tissues, with strong expression in testis, ovary, and uterus, and weak expression in heart and skeletal muscle.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
