PTPMT1 is a recently discovered dual specificity phosphatase that has been implicated in the regulation of insulin secretion.
Database analysis using the mouse ortholog, which encodes a deduced 261aa protein, revealed 60 orthologs from 4 different phylogenetic kingdoms that share more than 70% aa identity within the active site region.
The active site sequence between aa128 and aa148 of mouse and human PLIP is identical.
Northern blot analysis of several mouse tissues detected highest expression in testis.
Plip showed activity against PtdIns(5)P that was 44-fold greater than that against PtdIns(3,5)P2, its second most preferred substrate, when the phosphoinositides were presented in a lipid bilayer with phosphatidylserine
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
