Northern blot analysis revealed that HCAM3A is expressed as a 5.6-kb mRNA in heart and brain and at lower levels in several other tissues.
HCAM3B is expressed as an approximately 10-kb mRNA in brain and heart and, to a lesser extent, in lung.
The HCAM3B probe detected additional faint bands.
Although expression of full-length HCAM3A and HCAM3B in S.
cerevisiae did not result in PDE activity, an amino-truncated HCAM3A gave measurable PDE activity with high affinity for both cAMP and cGMP.consistent with the predicted mass of the PDE1C1 isoform, in human cardiac myocyte lysates.
Immunohistochemical analysis of human cardiac myocytes revealed PDE1C1 in a striated pattern, with strong staining of cardiac Z bands and weaker staining of M lines.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
