PCSH15 encodes a variety of isoforms with 3 to 11 ectodomains, a transmembrane domain, and a C-terminal cytoplasmic domain (CD).
The aa sequences of CD1, CD2, and CD3 are entirely different.
PCDH15-CD1 showed a limited pattern of expression and was detected in human testis, retina, and cochlea.
PCDH15-CD2 expression was present in the human heart, kidney, thymus, spleen, testis, retina and cochlea.
PCDH15-CD3 was widely transcribed and detected in almost all of 18 tissues tested.
PCDH15 expression in the inner and outer synaptic layers and the nerve fiber layer in human adult and fetal retinas.
Additional reactivity in the region of the outer limiting membrane/photoreceptor cell inner segments was observed in adult but not fetal retina.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-2ug/mlWestern Blot: 0.05-2µg/mlImmunohistochemistry (frozen): 5-20ug/mlImmunohistochemistry (paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PCDH15.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Protocadherin 15.
