(2003) identified a transcript that was downregulated in the nasopharyngeal carcinoma cell line HNE-1 relative to normal nasopharyngeal epithelial cells.
They assembled the full-length cDNA, designated NOR1, and found that it encodes a deduced 421aa protein with a predicted molecular mass of 48kD.
The protein contains an oxidored-nitro domain, 2 cAMP and cGMP-dependent protein kinase phosphorylation sites, 5 casein kinase II phosphorylation sites, and 4 N-myristoylation sites.
Northern blot analysis detected ubiquitous expression of 1.2- and 1.6-kb transcripts, with highest expression in skeletal muscle and heart.Nie et al.
(2003) determined that the NOR1 gene contains 10 exons and spans 33.4 kb.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
