An apical membrane Na+/H+ exchanger is involved in transepithelial, electroneutral Na+ absorption across renal and intestinal epithelia.
The apical Na+/H+ exchange activity is pharmacologically distinct from the basolateral Na+/H+ exchange activity, notably in terms of response to kinase regulation and in addition by the diuretic amiloride.
NHE1 (SLC9A1), the first of these exchangers to be cloned, localizes by immunocytochemical studies only to the basolateral membranes of polarized epithelial cells.
Transfection of OKP cells with dominant-negative PYK2 or small interfering PYK2 duplex RNA blocked acid activation of NHE3, whereas neither had an effect on glucocorticoid activation of NHE3.
Dominant-negative PYK2 also blocked acid activation of SRC kinase, which is required for acid regulation of NHE3.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
