(1996) cloned a novel Y-type receptor that they termed Y5 from rat hypothalamus and reported that it encodes a 456aa protein with less than 35% overall identity to known Y-type receptors.
They identified a human Y5 homolog in the GenBank database having 87% overall identity with rat Y5.
The rat and human Y5 protein sequences share 88% identity and show 30 to 33% identity to other NPY receptor subtypes.
Northern blot analysis revealed that NPY5R is expressed as a 2.6-kb mRNA in the brain but in no other tissues tested.
Hu et al.
(1996) also isolated another rat cDNA containing a 123aa insertion near the 5-prime end, which may represent an alternatively spliced form of the gene.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
