Myf5 belongs to a family of proteins known as myogenic regulatory factors (MRFs).
These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation.
MRF family members include Myf5, MyoD (Myf3), myogenin, and MRF4 (Myf6).
Sequence analysis and the pattern of expression of MYF5 in human muscle showed that MYF5, although structurally related to MyoD1, constitutes a different protein that is nevertheless capable of inducing the myogenic phenotype in embryonic C3H mouse fibroblasts.
In mice, the myogenic basic-helix-loop-helix genes are activated sequentially during the skeletal muscle developmental program.
Myf5 mRNA was first detected in the 8-day somite and was markedly reduced after day 14.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
