(1996) cloned MELK through the random sequencing of cDNA clones derived from a myeloid leukemic cell line.
The deduced 651aa protein shares 62% identity with Xenopus protein kinase p69Eg3.
Northern blot analysis detected expression in placenta, kidney, thymus, testis, ovary, and intestine.
Heyer et al.
(1997) cloned mouse Melk by differential display PCR analysis using cDNA from the egg and 2- and 8-cell-stage embryo cDNA libraries as template.
The deduced 644aa protein contains an N-terminal serine/threonine kinase domain that includes all 12 typical catalytic subdomains, and a leucine zipper motif.
The mouse and human proteins share 95% sequence identity in the kinase domain.
Northern blot analysis in mouse detected restricted expression to spermatogonia in testis and to oocytes in ovary.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
