Mannose-associated serine protease 1 (MASP1) is involved in the lectin pathway of the complement system and is responsible for cleaving C4 and C2 to form C4b2a, a C3-convertase.Mannose-binding lectin (MBL) is a serum component which participates in innate immunity by activating complement via a novel pathway.
Human MBL forms complexes with two types of serine proteases termed MASP (MBL-associated serine protease).
These two proteases, MASP1 and MASP2, are structurally similar to one another as well as to C1r and C1s.
Together, MASP, C1r and C1s constitute a novel serine protease family.
It is likely that human MASP1 is able to activate C3, while human MASP2 cleaves C4, although further functional studies are required to confirm this.
Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-2ug/mlWestern Blot: 1-5ug/mlImmunohistochemistry (frozen): 5-20ug/mlImmunohistochemistry (paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlELISA: 0.05-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human MASP1.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His20~Ala44 of MASP1 expressed in E. coli.
