A second EPO-independent peak of JUNB expression was associated with erythroid cell differentiation as measured by increased expression of erythroid-specific proteins.
More than 50% of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECSs), which may be required for effecting the proper transcriptional regulation of the gene.
Comparative sequence analyses involving kilobases of distal flanking DNAs for only a small number of vertebrate genes suggested that FECSs may emerge as common yet important functional components of genes.
If they account for a large fraction of the conserved sequence within a disease-related locus, then deleterious mutations might frequently occur in these regions and go undetected with current strategies.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.