Members of this family are characterized by a signal peptide, a B chain, a connecting C chain, and an A chain.
By EST database searching for sequences related to relaxin (RLN1), Conklin et al.
(1999) identified a cDNA encoding INSL5, also designated ZINS3.
The deduced 135aa protein, which shares 59% and 40% sequence identity with mouse Insl5 and human RLN1, respectively, has a dibasic cleavage site between the B and C chains.
Northern blot analysis revealed expression of an approximately 1.0kb transcript in uterus, rectum, and colon.
By radiation hybrid analysis and the use of surrounding markers, Conklin et al.
(1999) mapped the INSL5 gene to chromosome 1p31.1-p22.3.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
