Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.
Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology.
GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992).
By Northern blot analysis, Lau et al.
(1999) showed that GSK3B is predominantly expressed as 8.3- and 2.8kb transcripts in testis, thymus, prostate, and ovary, but weakly expressed in lung and kidney.
Western blot analysis revealed expression of a 46kD protein in lung, kidney, and brain.
By comparative analysis of various promoter constructs, Lau et al.
(1999) observed that GSK3B expression is greater in neuronal than in kidney cell lines.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
