Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes.
GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain.
GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines.
GATA3 was abundantly expressed in the T-lymphocyte lineage and was thought to participate in T-cell receptor gene activation through binding to enhancers.
Labastie et al.
(1994) cloned the human gene and the 5-prime end of the mouse gene.
The 2 zinc fingers of GATA3 are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
