Metazoan replication-dependent histone mRNAs end in a highly conserved stem-loop structure that is required for regulation of histone mRNA stability and is bound by stem loop-binding protein (SLBP).
Using the histone mRNA stem-loop sequence in RNA affinity purification assays, Dominski et al.
(2003) isolated HEXO (histone mRNA end-specific exonuclease) protein from HeLa whole-cell extracts.
They sequenced regions of HEXO protein using mass spectrometry and matched this protein to a full-length protein from a sequence database.
HEXO encodes a 50kD protein containing a SAP domain capable of recognizing AT-rich scaffold-attachment regions (SARs) in chromosomal DNA, and a exonuclease domain similar to those of DEDD exonuclease family members.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
