Eosinophil peroxidase is a haloperoxidase enzymeencoded by the EPX gene.
The enzyme is a heterodimeric 71-77kD peroxidase consisting of a heavier glycosylated chain and a lighter nonglycosylated chain.
In the presence of H2O2 formed by the eosinophil, and either chloride or bromide ions, eosinophil peroxidase provides a potent mechanism by which eosinophils kill multicellular parasites (such as, for example, the nematode worms involved in filariasis); and also certain bacteria (such as tuberculosis bacteria).
Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating hypobromite (hypobromous acid).
The enzyme is also capable of oxidizing thiocyanate (SCN-) and uses it as a co-substrate, with optimal concentrations occurring at about normal plasma levels.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin-300, 50% glycerol.
