The deduced 387aa human protein contains at least 1 transmembrane domain, 3 potential N-linked glycosylation sites, 6 potential phosphorylation sites, and a putative glycerol phospholipid domain found in acyltransferases.
Northern blot analysis revealed expression of 2.4- and 1.8-kb DGAT2 transcripts.
Highest expression was in liver and white adipose tissue, with lower levels in mammary gland, testis, and peripheral blood leukocytes.
Functional analysis confirmed DGAT activity, which was dependent on the presence of fatty acyl-CoA and diacylglycerol, in the membranes of DGAT2-expressing cells.
The authors found that DGAT2 functions in the presence of low levels of magnesium chloride, whereas DGAT1 functions best at higher levels of magnesium chloride.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
