Thymidylate kinase (EC 2.7.4.9; ATP:dTMP phosphotransferase) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.
Knowledge of the essential function of dTMP kinase in eukaryotes comes from the study of a cell cycle mutant, cdc8, in Saccharomyces cerevisiae.
The product of the cdc8 gene is involved in the elongation step of S phase in cell cycle progression.
Huang et al.
(1994) cloned the human thymidylate kinase gene by complementation with the cdc8 mutation.
The cDNA sequence revealed an open reading frame that encodes a protein with a molecular weight of 23,806.
Structural and functional analyses suggested that the cDNA codes for authentic human dTMP kinase.
The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DTYMK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to DTYMK (Pro43~Asp190) of His-tag, T7-tag at N-terminal, expressed in E. coli.
