CsA is an immunosuppressant drug widely used in organ transplantation to prevent rejection.
It reduces the activity of the immune system by interfering with the activity and growth of T cells.
Ciclosporin is synthesized by a nonribosomal peptide synthetase, ciclosporin synthetase.
The enzyme contains an adenylation domain, a thiolation domain, a condensation domain, and an N-methyltransferase domain.
The adenylation domain is responsible for substrate recognition and activation, whereas the thiolation domain covalently binds the adenylated aa to phosphopantetheine, and the condensation domain elongates the peptide chain.
Ciclosporin synthetase substrates include L-valine, L-leucine, L-alanine, glycine, 2-aminobutyric acid, 4-methylthreonine, and D-alanine, which is the starting aa in the biosynthetic process.
Applications: Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Frozen): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
