One of these was located between the gene encoding the putative subunit of the proteasome complex (MECL1) and a protein serine kinase gene.
The sequence of the protein was compared to protein sequences in the SWISSPROT database and identified as that of a chymotrypsin-like protease (CTRL).
This gene has 7 exons, covers about 2kb, and is transcribed in the same direction as the LCAT and the proteasome gene, from which it is immediately downstream.The open reading frame encodes a 264aa proenzyme that contains a putative leader sequence of 18aa.
The sequence is 54% identical to that of human chymotrypsin B.
Northern blot analysis showed that the gene is expressed specifically in the pancreas.
The substrate specificity of the mature enzyme was distinct from that of chymotrypsin B.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
