The deduced CILP2 protein shares 50.6% identity with CILP1, and both proteins have a central furin (FUR) endoprotease consensus cleavage site predicted to cause release of N- and C-terminal peptides.
The N-terminal halves of CILP1 and CILP2 contain a thrombospondin type-1 repeat and an immunoglobulin C2-type domain, but the N-terminal half of CILP2 lacks the putative aldehyde dehydrogenase cysteine active site and the ATP-binding site found in CILP1.
RT-PCR detected both CILP1 and CILP2 in cultured normal hyaline cartilage articular chondrocytes, but not in osteoblasts, fibroblasts, or vascular smooth muscle cells.
Only CILP1 was detected in normal knee meniscal fibrocartilage chondrocytic cells.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
