CDC7 is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity.
Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase.
It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression.
Overexpression of this gene product may be associated with neoplastic transformation for some tumors.
Additional transcript sizes have been detected, suggesting the presence of alternative splicing.
Cell division cycle 7 (CDC7) is a gene that codes for the protein Cdc7 kinase.
The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosmal DNA replication.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CDC7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CDC7 (Glu2~Thr232) with two N-terminal Tags, His-tag and T7-tag, expressed in E. coli.
