Cell division cycle protein 16 is a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase.
The APC complex is a cyclin degradation system that governs exit from mitosis.
Each component protein of the APC complex is highly conserved among eukaryotic organisms.
This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction.
Multiple alternatively spliced variants, encoding the same protein, have been identified.The predicted 619aa human CDC16 protein shares approximately 30% similarity to the yeast Cdc16 and S.
pombe cut9 proteins.
Antibodies against CDC16 detected a 72kD protein doublet on Western blots of human cell extracts.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
