CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells.
B-1 cells have limited diversity of their B-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive.
CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by a very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.
CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed.
In humans, the gene is located on the long arm of chromosome 11.
There is no confimed ligand for CD5.
T cells express higher levels of CD5 than B cells.
CD5 is upregulated on T cells upon strong activation.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
