CCKAR is a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones.
This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach.
In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine.
Ulrich et al.
(1993) used a combination of hybridization screening of a cDNA library and PCR to clone a 2.1-kb cDNA that encodes the human gallbladder CCK receptor type A (CCKAR).
Nucleotide sequence analysis revealed an open reading frame encoding a 428aa protein, with 7 putative transmembrane domains and a high degree of homology with the cholecystokinin A receptor protein of rat and guinea pig.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
