Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family.
The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus.
Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology.
Bax is a 21kD protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo.
Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-2ug/mlWestern Blot: 0.05-2µg/mlImmunohistochemistry (frozen): 5-20ug/mlImmunohistochemistry (paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Bax.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1~Gly192 of human Bcl2 Associated X Protein expressed in E. coli.
