The nucleotide sequence of the ATR gene showed extensive homology with the authentic AAT gene in the introns as well as exons.
The conservation of all RNA splice sites and lack of internal termination codons in the exons suggested that it may not be a classic pseudogene.
If expressed, it would result in a protein of 420aa residues, exhibiting a 70% overall homology with AAT.
The signal peptide sequence was well conserved, but the active site of protease inhibition (met-ser) in AAT had been changed to trp-ser.
The findings suggested that the putative protein is a secretory serine protease inhibitor with an altered substrate specificity.
Since even the introns showed 65% nucleotide sequence homology with the authentic AAT gene, ATR appears to have been derived from a recent duplication of the AAT gene.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ATR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ATR (Glu25~Tyr214) with two N-terminal Tags, His-tag and T7-tag, expressed in E. coli.
