(2006) observed a dose-responsive decrease in Aqp5 abundance in mouse lung epithelial cells exposed to hypotonic medium.
Hypotonic reduction of AQP5 was augmented and reduced, respectively, by conditions that activated or inhibited Trpv4.
Hypotonic reduction of AQP5 required extracellular calcium and was associated with increased intracellular calcium.
The response to hypotonicity was recapitulated by coexpression of TRPV4 and AQP5 in human embryonic kidney cells
Sidhaye et al.
(2006) concluded that AQP5 abundance is tightly controlled along a spectrum of extracellular osmolalities and that its abundance in hypotonic conditions can be regulated by TRPV4 activation.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
