Protein kinase C alpha (PKC-alpha) belongs to the Protein kinase C family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC-alpha is one of at least 12 different types of Protein kinase C identified in humans.
PKC-alpha is ubiquitously expressed and has roles in many different cellular processes including cell adhesion, cell transformation, proliferation, differentiation and cell cycle checkpoint.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 min is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 5% BSA, 0.9% sodium chloride, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% sodium azide. Reconsitute with 200ul sterile ddH2O.
Specificity：Recognizes human Protein kinase C alpha.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa153-182, MDHTEKRGRIYLKAEVADEKLHVTVRDAKN from the N-terminal of human Protein kinase C alpha. Species sequence homology: mouse, rat
