In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles.
The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation.
They are involved in signal transduction pathways in cooperation with HSP90.
They participate in all these processes through their ability to recognize nonnative conformations of other proteins.
They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, ~75kD band observed in lysates of cell lines HepG2, HeLa and MCF7 (calculated MW of 72.3kD according to NP_005338.1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human HSPA5.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the internal region, C-KVVEKKTKPYIQVD of human HSPA5 (NP_005338.1).
