This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes.
The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke.
The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates.
The gene has been associated with lung cancer risk.
A related family member, CYP1A2, is located approximately 25kb away from CYP1A1 on chromosome 15.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.3-1ug/ml, ~85-90kD band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kD according to NP_000490.1)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CYP1A1. Does not react with similiar P450.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to CQEKQLDENANVQ of human CYP1A1 (NP_000490.1).
