The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR).
Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition.
The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage.
In cortical thymocytes, CD3 is predominantly intra-cytoplasmic.
However, in medullary thymocytes, it appears on the T cell surface.
CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.3-1ug/ml, ~22kD band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kD according to NP_000724.1)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes the cytoplasmic domain (aa153-207) of human CD3E.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-RKGQRDLYSGLNQR of human CD3E(NP_000724.1). Species sequence homology: rat, porcine
