Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene.
AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence.
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells.
Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation.
In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.
A related pseudogene has been identified on chromosome 10.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.01-0.1ug/ml, ~70kD band observed in Mouse and Rat Heart and Kidney lysates, and approx.
65kD in Pig Heart lysates (calculated MW of 66.8kD according to Mouse NP_036149, Rat NP_112646.1 and Pig NP_001284561.1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human AIFM1. Species Crossreactivity: mouse, rat, porcine. Expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-NEVAKLFNIHED of human AIFM1 at C-terminal. Species sequence homology: canine, bovine
