TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins.
The most abundant isoform, TGF-beta-1, is a 25kD homodimer composed of two 12.5kD subunits joined by disulfide bonds.
TGF-beta-1 is a highly conserved molecule-the amino acid sequence between human and mouse differ only by one residue.
Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types.
It is produced by a wide variety of cell and tissue types during all stages of cell differentiation.
TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.
Applications:Suitable for use in ELISA, Neutralization, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cellsWestern Blot: 2ug/ml using Saos-2 human osteosarcoma cell lineNeutralization: Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line.
Tsang, M.
et al.
(1995) Cytokine 7:389.
The Neutralization Dose (ND50) is typically 0.4-2ug/mL in the presence of 500ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL TGF‑ beta 1
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human LAP TGF‑ beta 1.
Isotype：IgG
Calc Applications Abbrev：E IC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu30-Ser390 from human LAP TGF‑ beta 1, expressed in S. frugiperda insect ovarian cell line Sf 21 and CHO cells.
