The neurofibrillary tangles (NFT) are neuronal inclusions that are the hallmark lesions of Alzheimer’s disease (AD).
These tangles are made up of paired helical filaments (PHF).
The PHF are highly insoluble structures and are known to be composed of a highly phosphorylated form of the microtubule-associated protein tau and may contain associated lipid.
Insoluble NFT which were extracted in boiling sodium dodecyl sulfate, purified by sucrose gradient centrifugation and disaggregated by sonication were used as an immunogen for the preparation of this antiserum.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 1:500Immunohistochemistry (frozen): 1:1000Immunohistochemistry (paraffin): 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
