Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages.
It is estimated that as much as 1% of consumed oxygen may be converted to ROS, which can cause damage to various cellular components.
Proteins are one of the cellular components most vulnerable to oxidative damage by ROS, which results in an increase in protein carbonyl content.
This oxidative modification of proteins can lead to cross-linking, peptide fragmentation, modified residues and the conversion of one amino group to another.
If sufficient protein damage accumulates, cell death will occur.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
