Beta-galactosidase, also called beta-gal or Beta-gal, is a hydrolase enzyme that catalyzes the hydrolysis of Beta-galactosides into monosaccharides.
Substrates of different Beta-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
Lactase is often confused as an alternative name for Beta-galactosidase, but it is actually simply a sub-class of Beta-galactosidase.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,0000Western Blot: 1:5000-1:10,000Immunohistochemistry: 1:1500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5mg
Host：rabbit
Source Antibody：E. coli
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes E. coli beta galactosidase.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ec
Immunogen：Full length native beta galactosidase isolated from E. coli.
