Beta-galactosidase, also called beta-gal or Beta-gal, is a hydrolase enzyme that catalyzes the hydrolysis of Beta-galactosides into monosaccharides.
Substrates of different Beta-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
Lactase is often confused as an alternative name for Beta-galactosidase, but it is actually simply a sub-class of Beta-galactosidase.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000Western Blot: 1:2000-1:10,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:rabbit
Grade:Highly Purified
Purity:Purified by Ion Exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide.
Specificity:Recognizes E. coli beta galactosidase.
Isotype:IgG
Calc Applications Abbrev:E IF WB
Calc Crossreactivity:Ec
Immunogen:Highly purified beta galactosidase from E. coli