The amyloid precursor protein (APP) is a large membrane protein whose C-terminus projects into the extracellular space.
In Alzheimer's disease (AD), the APP is proteolytically cleaved at the N-terminal of Ab by b-secretase (BACE) to release a ~100kD APPsb protein into the extracellular space.
The remaining 12 kDa fragment remains membrane bound where it can be cleaved at its C-terminus by g-secretase (presenilins) to release the insoluble Ab peptide into the extracellular space with the ~8kD APP C-terminal fragment (CTFb) remaining membrane bound.
The APP is the subject of intensive investigations to determine how this protein is broken down abnormally in AD brains to give rise to Ab, which is present in senile plaques and vessels
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:25,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservative added.
Specificity：Recognizes human APP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic eptide corresponding to GYENPTYKFFEQMQN from human APP.
