Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion.
It is basically a phosphomonoesterase.
It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function; therefore, it has an acid pH optimum.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:40,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
