84-4443-74　TOE1, CT (Target of EGR1 Protein 1) 100ul　367000

特徴

• Target of EGR1 protein 1 (UniProt Q96GM8) is encoded by the TOE1 gene (Gene ID 114034) in human.
• Originally characterized and thereby named as a downstream target of the immediate early gene Egr1, TOE1 is a nuclear protein localized primarily in nucleoli and Cajal bodies.
• TOE1 overexpression causes an upregulation of the cyclin-dependent kinase inhibitor p21 and cell cycle arrest at G2/M.
• TOE1 also interacts wtih p53 C-terminal tetramerization domain and enhances p53 transcriptional activity.
• In addtion, TOE1 exhibits inhibitory activity against HIV-1 replication and evidence indicates TOE1 interaction with the viral specific transactivator response element as part of the inhibitory mechanism.
• TOE1 is upregulated and secreted by activated CD8(+) T lymphocytes, and TOE1 can be proeolytically cleaved by granzyme B at D328 and D363, generating a 35aa (aa329-363) fragment containing the nuclear localization signal/NLS (aa335-347).
• The 35aa fragment retains both the antiviral potency and cell-penetrating capability.
• Human TOE1 is produced as a 510aa protein, containing a C3H1-type Zinc finger (aa294-322) and anuclear localization signal/NLS (a.a.335-347).
• The initiation methionine is removed posttranslationally.
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Immunoprecipitation: 1ul immunoprecipitated TOE1 from 20ug of HEK293T cell lysate Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.