84-4443-73　TIAM1 (TIAM-1, T-lymphoma Invasion and Metastasis-inducing Protein 1) 100ug　366999

特徴

• T-lymphoma invasion and metastasis-inducing protein 1 (UniProt Q13009; also known as TIAM-1) is encoded by the TIAM1 gene (Gene ID 7074) in human.
• TIAM-1 is a ubiquitously expressed guanine nucleotide exchange factor (GEF) that specifically activates the Rho-like GTPase Rac1 in response to growth factors and cell-substrate interaction.
• TIAM-1 associates with GTP-bound Ras and functions as an effector that mediates Rac activation by Ras.
• TIAM-1 promotes the formation of adherens junctions and the induction of an epithelial-like phenotype in mesenchymal cells, and is essential for the formation and maintenance of cadherin-based adhesions.
• Tiam1-knockdown in mice protects against Ras-induced skin carcinogenesis as a result of increased apoptosis and reduced proliferation/growth of keratinocytes.
• However, the fewer skin tumors developed progress more frequently to malignancy in Tiam1-knockout mice, indicating that loss of Tiam1 stimulates malignant conversion.
• Cellular TIAM-1 is targeted for proteasome-dependent degradation by the SCFbTrCP ubiquitin ligase in cooperation with casein kinase 1 and the MEK/ERK pathway upon mitogenic stimulation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mL detects TIAM-1 in 10ug of Neuro-2a mouse neuroblastoma cell lysateOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.