Pulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human.
Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension.
Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D.
SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity.
SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus.
SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles.
SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival.
In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas.
Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis.
Human SP-D is produced with a signal peptide sequence (aa1-20), which is removed posttranslationally to yield the mature protein (aa21-375) that contains a collagen-like domain (aa46-222), a coiled coil region (aa223-252), and a C-type lectin domain (aa260-375).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:1000 detects surfactant protein D in human lung and pancreas tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Surfactant Protein D.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D, KLH conjugated.
