84-4443-69　RTN3 (Reticulon-3, HAP, Homolog of ASY Protein, Neuroendocrine-specific Protein-like 2, Neuroendocrine-specific Protein-like II, NSP-like Protein 2, NSP-like Protein II, NSPLII) [KO Validated] 100ul　366989

特徴

• Reticulon-3 (UniProt O95197; also known as HAP, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, NSP-like protein 2, NSP-like protein II, NSPLII) is encoded by the RTN3 (also known as ASYIP, NSPL2) gene (Gene ID 10313) in human.
• Reticulon-3/RTN3 belongs to the RTN family of proteins characterized by their C-terminal reticulon homolog domain (RHD; aa844-1032 in RTN3).
• There exist four mammalian reticulons (RTN1-4), with each member having multiple spliced isoforms.
• RTN3 is expressed in neurons and localized in axons and growth cones.
• RTN3 overexpression in neurons results in reduced amyloid deposition in murine models of Alzheimer's disease (AD).
• Consistently, RTN3 deficiency elevates BACE1 protein level and enhances amyloid deposition in AD mice that express human mutant APP and presenilin-1 transgenes.
• Human RTN3 is a multipass ER and golgi membrane protein with 3 membrane helical regions (aa864-997, 948-968, 973-993) and four cytoplasmic regions (aa2-863, 888-947, 969-972, 994-1032).
• Its C-terminal RHD mediates interaction with FADD (aa987-1032) and BACE1 (aa1000-1002).
• Alternative splicings generate 7 isoforms (UniProt O95197-1 througth O95197-7).
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:2000 immunostained dystrophic neurites in Alzheimer's diseased (AD) human frozen brain tissue sections by fluorescent immunohistochemistryWestern Blot: 1:2000 detects RTN3 isoforms in 50ug of brain tissue lysate from a wild-type, but not RTN3-knockout, mouseImmunohistochemistry: 1:2000 immunostained dystrophic neurites in Alzheimer's diseased (AD) human frozen brain tissue sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.